While the importance of choosing the appropriate reference genes has been widely recognized, researchers often rely on knowledge over experience and often simply choose to use classical reference genes (GAPDH, B2M, ACTB, TBP, and the like). In order to perform an accurate analysis of relative gene expression in different samples, tissues, or experimental conditions, reference genes must be used to normalize variation across all samples. The company develops, manufactures, and markets a broad range of products for the life science research and clinical diagnostic markets.Quantitative real-time PCR is one of the most important methods for rapid and reliable quantification of gene expression. “The versatile CFX Duet System allows users to quickly get started with gene expression, genotyping, quantification and thermal gradient optimization, as well as protein characterization using FRET.” “We are pleased to expand our portfolio of qPCR instruments with the CFX Duet System to help customers run singleplex and duplex assays with a software that matches the robust thermal capabilities of our premium CFX Opus formats,” says Steven Blakely, director for gene expression and software technology, Bio-Rad. The CFX Duet PCR System utilizes CFX Maestro Software for easy experimental setup and analysis, and to deliver customized reports without exporting data to other programs. The CFX Duet is a two-color system that is factory calibrated for common dyes and, without the need for passive reference dyes, allows the precise quantification of up to two targets in genotyping and multiple gene expression analyses.Īn additional fluorescence resonance energy transfer (FRET) mode supports protein melt analysis for basic protein characterization. The CFX Duet PCR System offers the robust thermal performance and proprietary, accurate optical shuttle system of Bio-Rad’s CFX Opus System, with thermal gradient functionality to enable optimization in fewer runs. Bio-Rad Laboratories, a global leader of life science research and clinical diagnostic products, announced the launch of the CFX Duet Real-Time PCR System to support researchers in developing singleplex and duplex quantitative PCR (qPCR) assays.

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